Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor
- Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells.
Verfasserangaben: | Antje Katharina Heinrich, Angela Glaeser, Nicholas J. TobiasORCiD, Ralf Heermann, Helge Björn BodeORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-436782 |
DOI: | https://doi.org/10.1016/j.heliyon.2016.e00197 |
ISSN: | 2405-8440 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/27957552 |
Titel des übergeordneten Werkes (Englisch): | Heliyon |
Verlag: | Elsevier |
Verlagsort: | London [u. a.] |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 26.06.2017 |
Jahr der Erstveröffentlichung: | 2016 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 26.06.2017 |
Freies Schlagwort / Tag: | Cell biology; Genetics; Microbiology |
Jahrgang: | 2 |
Ausgabe / Heft: | 11, e00197 |
Seitenzahl: | 32 |
Erste Seite: | 1 |
Letzte Seite: | 32 |
Bemerkung: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
HeBIS-PPN: | 428726437 |
Institute: | Biowissenschaften / Biowissenschaften |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Lizenz (Deutsch): | Creative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0 |