Größenbestimmung biologischer Partikel durch Gelchromatographie an Sepharose 6B
Determination of particle size by gel chromatography with Sepharose 6B
- Gel chromatography with 6% agarose gel (Sepharose 6B) can be used for measuring the Stokes’ radius of biological particles within the range of 1,5 nm and 35 nm. The molecular weight determination of proteins is not very reliable with this method. Hepatitis sera have been chromatographied to measure the size of hepatitis associated antigen (Australia-antigen). The radius of this antigen was determined to be 10,3 nm, which agrees with the results of electron microscopy and ultracentrifugation. The Stokes’ radius of human serum IgM was found to be 10,6 nm.
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| Author: | Wolfram H. GerlichORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-698610 |
| DOI: | https://doi.org/10.1515/znb-1971-1017 |
| ISSN: | 1865-7117 |
| ISSN: | 0932-0776 |
| Parent Title (German): | Zeitschrift für Naturforschung, B |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | German |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1971 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/07/05 |
| Volume: | 26 |
| Issue: | 10 |
| Page Number: | 5 |
| First Page: | 1040 |
| Last Page: | 1044 |
| HeBIS-PPN: | 511915055 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
