Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules
- Vacuolar proton-translocating ATPase (holoATPase and free membrane sector) was isolated from bovine chromaffin granules by blue native polyacrylamide gel electrophoresis. A 5-fold excess of membrane sector over holoenzyme was determined in isolated chromaffin granule membranes. M9.2, a novel extremely hydrophobic 9.2-kDa protein comprising 80 amino acids, was detected in the membrane sector. It shows sequence and structural similarity to Vma21p, a yeast protein required for assembly of vacuolar ATPase. A second membrane sector-associated protein (M8-9) was identified and characterized by amino-terminal protein sequencing.
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| Author: | Jürgen Ludwig, Stefan Kerscher, Ulrich BrandtORCiDGND, Kathy Pfeiffer, Fariha Getlawi, David K. Apps, Hermann Schägger |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-758705 |
| DOI: | https://doi.org/10.1074/jbc.273.18.10939 |
| ISSN: | 0021-9258 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/9556572 |
| Parent Title (English): | Journal of biological chemistry |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Year of first Publication: | 1998 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/04/25 |
| Volume: | 273.1998 |
| Issue: | 18 |
| Page Number: | 9 |
| First Page: | 10939 |
| Last Page: | 10947 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
