hFRUIT: an optimized agent for optical clearing of DiI-stained adult human brain tissue
- Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
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| Author: | Sven Hildebrand, Anna Schueth, Klaus von Wangenheim, Christian Mattheyer, Francesco PampaloniORCiDGND, Hansjürgen BratzkeGND, Alard F. RoebroeckORCiD, Ralf GaluskeGND |
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| URN: | urn:nbn:de:hebis:30:3-547728 |
| DOI: | https://doi.org/10.1038/s41598-020-66999-3 |
| ISSN: | 2045-2322 |
| Parent Title (English): | Scientific reports |
| Publisher: | Nature Publishing Group |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/06/19 |
| Date of first Publication: | 2020/06/19 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/06/25 |
| Issue: | Article number 9950 |
| HeBIS-PPN: | 467145806 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Medizin / Medizin | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
