The non-structural proteins NS3 and NS5A of Hepatitis C Virus (HCV) are degraded by two host proteolysis systems
- Each lifecycle of the Hepatitis C virus (HCV) produces structural and non-structural (NS) proteins in equimolar. Structural proteins were either assembled or degraded by host proteolysis systems, while NS proteins remain inside the host cells and don’t accumulate. Therefore, they must be degraded. Here, NS3 and NS5A half-lives were quantified in the presence of autolysosome and proteasome different modulators. Inhibitors of both systems increased the half-life, while inducers decreased the half-life. Furthermore, polyubiquitination of NS3 and NS5A was observed. Additionally, their intracellular co-localization with autolysosome (LAMP2) and proteasome (PSMB5) was observed, and inhibitors of both systems increased the degree of co-localization. A better understanding of NS protein degradation might help to improve medical interventions during HCV infections in the future.
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| Verfasserangaben: | Sara MohamedORCiDGND, Jörg SoppaORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-754170 |
| URL: | https://www.biorxiv.org/content/10.1101/2023.08.16.553615v1 |
| DOI: | https://doi.org/10.1101/2023.08.16.553615 |
| Titel des übergeordneten Werkes (Englisch): | bioRxiv |
| Dokumentart: | Preprint |
| Sprache: | Englisch |
| Datum der Veröffentlichung (online): | 17.08.2023 |
| Datum der Erstveröffentlichung: | 17.08.2023 |
| Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Datum der Freischaltung: | 01.09.2023 |
| Freies Schlagwort / Tag: | HCV; NS3; NS5A; autophagy; non-structural proteins; proteasome; protein degradation; proteolysis systems |
| Ausgabe / Heft: | 2023.08.16.553615 Version 1 |
| Auflage: | Version 1 |
| Seitenzahl: | 30 |
| Institute: | Biowissenschaften / Biowissenschaften |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Sammlungen: | Universitätspublikationen |
| Lizenz (Deutsch): |  Creative Commons - CC BY-ND - Namensnennung - Keine Bearbeitungen 4.0 International |
