Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
- Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.
Author: | Sandra Siegert, Sonja Thaler, Ralf Wagner, Barbara SchnierleORCiDGND |
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URN: | urn:nbn:de:hebis:30-26194 |
URL: | http://www.aidsrestherapy.com/content/2/1/7 |
Parent Title (German): | AIDS Research and Therapy |
Document Type: | Article |
Language: | English |
Year of Completion: | 2005 |
Date of first Publication: | 2005/09/12 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2006/05/08 |
Tag: | HIV-1; MLV/HIV-1; pseudotyped vectors |
Volume: | 2 |
Issue: | 7 |
Page Number: | 6 |
Note: | © 2005 Siegert et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Source: | AIDS Res Ther. 2005; 2: 7. http://www.aidsrestherapy.com/content/2/1/7 |
HeBIS-PPN: | 189203684 |
Institutes: | Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Licence (German): | Creative Commons - Namensnennung 2.0 |