A fast and efficient translational control system for conditional expression of yeast genes
- A new artificial regulatory system for essential genes in yeast is described. It prevents translation of target mRNAs upon tetracycline (tc) binding to aptamers introduced into their 5'UTRs. Exploiting direct RNA–ligand interaction renders auxiliary protein factors unnecessary. Therefore, our approach is strain independent and not susceptible to interferences by heterologous expressed regulatory proteins. We use a simple PCR-based strategy, which allows easy tagging of any target gene and the level of gene expression can be adjusted due to various tc aptamer-regulated promoters. As proof of concept, five differently expressed genes were targeted, two of which could not be regulated previously. In all cases, adding tc completely prevented growth and, as shown for Nop14p, rapidly abolished de novo protein synthesis providing a powerful tool for conditional regulation of yeast gene expression.
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| Author: | Peter KötterORCiD, Julia E. WeigandORCiDGND, Britta Meyer, Karl-Dieter Entian, Beatrix SüßGND |
|---|---|
| URN: | urn:nbn:de:hebis:30-80630 |
| DOI: | https://doi.org/10.1093/nar/gkp578 |
| ISSN: | 0301-5610 |
| ISSN: | 0305-1048 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/19592423 |
| Parent Title (English): | Nucleic acids research |
| Publisher: | Oxford Univ. Press |
| Place of publication: | Oxford |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2009/07/10 |
| Date of first Publication: | 2009/07/10 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2010/09/24 |
| Volume: | 37 |
| Issue: | (18): e120 |
| Page Number: | 7 |
| First Page: | 1 |
| Last Page: | 7 |
| Note: | © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Source: | Nucleic acids research, Vol. 37, No. 18 e120 ; doi:10.1093/nar/gkp578 ; http://nar.oxfordjournals.org/content/37/18/e120.abstract |
| HeBIS-PPN: | 228850614 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Sammlung Biologie / Sondersammelgebiets-Volltexte | |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell 2.0 |
