Direct evidence that the N-terminal extensions of the TAP complex act as autonomous interaction scaffolds for the assembly of the MHC I peptide-loading complex

The loading of antigenic peptides onto major histocompatibility complex class I (MHC I) molecules is an essential step in the adaptive immune response against virally or malignantly transformed cells. The ER-resident pep
The loading of antigenic peptides onto major histocompatibility complex class I (MHC I) molecules is an essential step in the adaptive immune response against virally or malignantly transformed cells. The ER-resident peptide-loading complex (PLC) consists of the transporter associated with antigen processing (TAP1 and TAP2), assembled with the auxiliary factors tapasin and MHC I. Here, we demonstrated that the N-terminal extension of each TAP subunit represents an autonomous domain, named TMD0, which is correctly targeted to and inserted into the ER membrane. In the absence of coreTAP, each TMD0 recruits tapasin in a 1:1 stoichiometry. Although the TMD0s lack known ER retention/retrieval signals, they are localized to the ER membrane even in tapasin-deficient cells. We conclude that the TMD0s of TAP form autonomous interaction hubs linking antigen translocation into the ER with peptide loading onto MHC I, hence ensuring a major function in the integrity of the antigen-processing machinery.
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Metadaten
Author:Sabine Hulpke, Maiko Tomioka, Elisabeth Kremmer, Kazumitsu Ueda, Rupert Abele, Robert Tampé
URN:urn:nbn:de:hebis:30:3-288735
DOI:http://dx.doi.org/10.1007/s00018-012-1005-6
ISSN:1420-9071
ISSN:1420-682X
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=22638925
Parent Title (English):Cellular and molecular life sciences : (CMLS)
Publisher:Springer
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2012/05/27
Date of first Publication:2012/05/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/02/14
Volume:69
Pagenumber:11
First Page:3317
Last Page:3327
Note:
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
HeBIS PPN:331500736
Institutes:Biochemie und Chemie
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 3.0

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