Use of axonal projection patterns for the homologisation of cerebral nerves in Opisthobranchia, Mollusca and Gastropoda

Introduction: Gastropoda are guided by several sensory organs in the head region, referred to as cephalic sensory organs (CSOs). These CSOs are innervated by distinct nerves. This study proposes a unified terminology for
Introduction: Gastropoda are guided by several sensory organs in the head region, referred to as cephalic sensory organs (CSOs). These CSOs are innervated by distinct nerves. This study proposes a unified terminology for the cerebral nerves and the categories of CSOs and then investigates the neuroanatomy and cellular innervation patterns of these cerebral nerves, in order to homologise them. The homologisation of the cerebral nerves in conjunction with other data, e.g. ontogenetic development or functional morphology, may then provide insights into the homology of the CSOs themselves.
Results: Nickel-lysine axonal tracing (“backfilling”) was used to stain the somata projecting into specific nerves in representatives of opisthobranch Gastropoda. Tracing patterns revealed the occurrence, size and relative position of somata and their axons and enabled these somata to be mapped to specific cell clusters. Assignment of cells to clusters followed a conservative approach based primarily on relative location of the cells. Each of the four investigated cerebral nerves could be uniquely identified due to a characteristic set of soma clusters projecting into the respective nerves via their axonal pathways.
Conclusions: As the described tracing patterns are highly conserved morphological characters, they can be used to homologise nerves within the investigated group of gastropods. The combination of adequate number of replicates and a comparative approach allows us to provide preliminary hypotheses on homologies for the cerebral nerves. Based on the hypotheses regarding cerebral nerve homology together with further data on ultrastructure and immunohistochemistry of CSOs published elsewhere, we can propose preliminary hypotheses regarding homology for the CSOs of the Opisthobranchia themselves.
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Metadaten
Author:Annette Klussmann-Kolb, Roger P. Croll, Sid Staubach
URN:urn:nbn:de:hebis:30:3-295199
DOI:http://dx.doi.org/10.1186/1742-9994-10-20
ISSN:1742-9994
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=23597272
Parent Title (English):Frontiers in zoology
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2013
Date of first Publication:2013/04/18
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2013/04/26
Tag:Axonal tracing; CSO; Cephalic sensory organs; Cerebral nerves; Homology; Opisthobranchia
Volume:10
Issue:20
Pagenumber:13
Note:
© 2013 Klussmann-Kolb et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS PPN:338318445
Institutes:Biowissenschaften
Institut für Ökologie, Evolution und Diversität
Dewey Decimal Classification:570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 2.0

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