Wilfried Posch, Marion Steger, Ulla Knackmuss, Michael Blatzer, Hanna-Mari Baldauf, Wolfgang Doppler, Tommy E. White, Paul Hörtnagl, Felipe Diaz-Griffero, Cornelia Lass-Flörl, Hubert Hackl, Arnaud Moris, Oliver Till Keppler, Doris Wilflingseder
- DCs express intrinsic cellular defense mechanisms to specifically inhibit HIV-1 replication. Thus, DCs are productively infected only at very low levels with HIV-1, and this non-permissiveness of DCs is suggested to go along with viral evasion. We now illustrate that complement-opsonized HIV-1 (HIV-C) efficiently bypasses SAMHD1 restriction and productively infects DCs including BDCA-1 DCs. Efficient DC infection by HIV-C was also observed using single-cycle HIV-C, and correlated with a remarkable elevated SAMHD1 T592 phosphorylation but not SAMHD1 degradation. If SAMHD1 phosphorylation was blocked using a CDK2-inhibitor HIV-C-induced DC infection was also significantly abrogated. Additionally, we found a higher maturation and co-stimulatory potential, aberrant type I interferon expression and signaling as well as a stronger induction of cellular immune responses in HIV-C-treated DCs. Collectively, our data highlight a novel protective mechanism mediated by complement opsonization of HIV to effectively promote DC immune functions, which might be in the future exploited to tackle HIV infection.
MetadatenAuthor: | Wilfried Posch, Marion Steger, Ulla Knackmuss, Michael Blatzer, Hanna-Mari Baldauf, Wolfgang Doppler, Tommy E. White, Paul Hörtnagl, Felipe Diaz-Griffero, Cornelia Lass-Flörl, Hubert Hackl, Arnaud Moris, Oliver Till KepplerORCiDGND, Doris Wilflingseder |
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URN: | urn:nbn:de:hebis:30:3-380087 |
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DOI: | https://doi.org/10.1371/journal.ppat.1005005 |
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ISSN: | 1553-7374 |
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ISSN: | 1553-7366 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26121641 |
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Parent Title (English): | PLoS pathogens |
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Publisher: | PLoS |
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Place of publication: | Lawrence, Kan. |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2015/06/29 |
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Date of first Publication: | 2015/06/29 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2015/09/06 |
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Volume: | 11 |
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Issue: | (6): e1005005 |
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Page Number: | 23 |
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First Page: | 1 |
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Last Page: | 23 |
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Note: | Copyright: © 2015 Posch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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HeBIS-PPN: | 370785959 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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