Nueva técnica de microscopía electrónica de barrido para el estudio de Coccoidea (Insecta: Hemiptera)
- A new protocol to prepare material of Coccoidea (Insecta: Hemiptera) for observation with scanning electron microscopy is presented. This technique consists of cleaning samples (stored in 96% alcohol) with acetone and xylene, taking them directly to the hydration step, and subsequently dehydrating them in alcohol. Fixation in glutaraldehyde and post-fi xation in osmium tetroxide are omitted. The cleaning of the samples and the correct critical point dryer are very important.
Author: | Andrea Saracho Bottero, Lucía E. Claps |
---|---|
URN: | urn:nbn:de:hebis:30:3-396957 |
ISSN: | 1942-1354 |
Parent Title (English): | Insecta mundi : a journal of world insect systematics |
Series (Serial Number): | Insecta Mundi (0446) |
Publisher: | Center for Systematic Entomology |
Place of publication: | Gainesville, Fla. |
Document Type: | Part of Periodical |
Language: | English |
Date of Publication (online): | 2016/05/19 |
Year of first Publication: | 2015 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2016/05/19 |
Tag: | Dactylopiidae; Pseudococcidae; SEM |
Page Number: | 8 |
First Page: | 1 |
Last Page: | 5 |
HeBIS-PPN: | 380636956 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie) |
Sammlungen: | Sammlung Biologie / Sondersammelgebiets-Volltexte |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |