In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells

  • Background: Electrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation. Methods: In the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points. Results: Results showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES. Discussion: This study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.

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Author:Sahba Mobini, Liudmila LeppikORCiDGND, Vishnu Thottakkattumana Parameswaran, John Howard BarkerORCiD
URN:urn:nbn:de:hebis:30:3-442273
DOI:https://doi.org/10.7717/peerj.2821
ISSN:2167-8359
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28097053
Parent Title (English):PeerJ
Publisher:PeerJ, Inc.
Place of publication:London [u. a.]
Contributor(s):Jalees Rehman
Document Type:Article
Language:English
Date of Publication (online):2017/06/19
Date of first Publication:2017/01/12
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/06/19
Tag:Adipose tissue-derived mesenchymal stem cells; Bone marrow-derived mesenchymal stem cells; Bone tissue engineering; Direct current electrical stimulation
Volume:5
Issue:e2821
Page Number:15
First Page:1
Last Page:15
Note:
Distributed under Creative Commons CC-BY 4.0
HeBIS-PPN:425035646
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0