When genomics is not enough : experimental evidence for a decrease in LINE-1 activity during the evolution of Australian marsupials
- he autonomous transposable element LINE-1 is a highly abundant element that makes up between 15% and 20% of therian mammal genomes. Since their origin before the divergence of marsupials and placental mammals, LINE-1 elements have contributed actively to the genome landscape. A previous in silico screen of the Tasmanian devil genome revealed a lack of functional coding LINE-1 sequences. In this study we present the results of an in vitro analysis from a partial LINE-1 reverse transcriptase coding sequence in five marsupial species. Our experimental screen supports the in silico findings of the genome-wide degradation of LINE-1 sequences in the Tasmanian devil, and identifies a high frequency of degraded LINE-1 sequences in other Australian marsupials. The comparison between the experimentally obtained LINE-1 sequences and reference genome assemblies suggests that conclusions from in silico analyses of retrotransposition activity can be influenced by incomplete genome assemblies from short reads.
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| Author: | Susanne GallusGND, Fritjof LammersORCiDGND, Maria Anna NilssonORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-443058 |
| DOI: | https://doi.org/10.1093/gbe/evw159 |
| ISSN: | 1759-6653 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27389686 |
| Parent Title (English): | Genome biology and evolution |
| Publisher: | Oxford Univ. Press |
| Place of publication: | Oxford |
| Contributor(s): | Ellen Pritham |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2017/06/26 |
| Year of first Publication: | 2016 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2017/06/26 |
| Tag: | LINE-1; Tasmanian devil; marsupials; retrotransposition; transposable element |
| Volume: | 8 |
| Issue: | 8 |
| Page Number: | 7 |
| First Page: | 2406 |
| Last Page: | 2412 |
| Note: | The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| HeBIS-PPN: | 424985152 |
| Institutes: | Biowissenschaften / Institut für Ökologie, Evolution und Diversität |
| Fachübergreifende Einrichtungen / Biodiversität und Klima Forschungszentrum (BiK-F) | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (English): |  Creative Commons - Namensnennung-Nicht kommerziell 4.0 |
