Strategies in megasynthase engineering – fatty acid synthases (FAS) as model proteins
- Megasynthases are large multienzyme proteins that produce a plethora of important natural compounds by catalyzing the successive condensation and modification of precursor units. Within the class of megasynthases, polyketide synthases (PKS) are responsible for the production of a large spectrum of bioactive polyketides (PK), which have frequently found their way into therapeutic applications. Rational engineering approaches have been performed during the last 25 years that seek to employ the "assembly-line synthetic concept" of megasynthases in order to deliver new bioactive compounds. Here, we highlight PKS engineering strategies in the light of the newly emerging structural information on megasynthases, and argue that fatty acid synthases (FAS) are and will be valuable objects for further developing this field.
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| Author: | Manuel Fischer, Martin GriningerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-444542 |
| DOI: | https://doi.org/10.3762/bjoc.13.119 |
| ISSN: | 1860-5397 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/28694866 |
| Parent Title (English): | Beilstein journal of organic chemistry |
| Publisher: | Beilstein-Institut zur Förderung der Chemischen Wissenschaften |
| Place of publication: | Frankfurt, Main |
| Contributor(s): | J. S. Dickschat |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2017/09/04 |
| Date of first Publication: | 2017/06/21 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2017/09/04 |
| Tag: | fatty acid synthases; megasynthases; metabolic enzyme engineering; polyketide synthases; protein design |
| Volume: | 13 |
| Page Number: | 8 |
| First Page: | 1204 |
| Last Page: | 1211 |
| Note: | This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| HeBIS-PPN: | 424938812 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
