RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model
- Mitochondrial respiratory supercomplexes (mtRSCs) are stoichiometric assemblies of electron transport chain (ETC) complexes in the inner mitochondrial membrane. They are hypothesized to regulate electron flow, the generation of reactive oxygen species (ROS) and to stabilize ETC complexes. Using the fungal ageing model Podospora anserina, we investigated the impact of homologues of the Saccharomyces cerevisiae respiratory supercomplex factors 1 and 2 (termed PaRCF1 and PaRCF2) on mtRSC formation, fitness and lifespan. Whereas PaRCF2’s role seems negligible, ablation of PaRCF1 alters size of monomeric complex IV, reduces the abundance of complex IV-containing supercomplexes, negatively affects vital functions and shortens lifespan. PaRcf1 overexpression slightly prolongs lifespan, though without appreciably influencing ETC organization. Overall, our results identify PaRCF1 as necessary yet not sufficient for mtRSC formation and demonstrate that PaRCF1-dependent stability of complex IV and associated supercomplexes is highly relevant for maintenance of the healthy lifespan in a eukaryotic model organism.
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| Author: | Fabian Fischer, Christodoulos Filippis, Heinz D. OsiewaczORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-451187 |
| DOI: | https://doi.org/10.1038/srep12697 |
| ISSN: | 2045-2322 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26220011 |
| Parent Title (English): | Scientific reports |
| Publisher: | Nature Publishing Group |
| Place of publication: | London |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2015 |
| Year of first Publication: | 2015 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2017/11/21 |
| Tag: | Biochemistry; Mitochondria |
| Volume: | 5 |
| Issue: | Art. 12697 |
| Page Number: | 13 |
| First Page: | 1 |
| Last Page: | 13 |
| Note: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| HeBIS-PPN: | 424950928 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Biowissenschaften |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
