The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs

  • The nuclear exosome and its essential co-factor, the RNA helicase MTR4, play crucial roles in several RNA degradation pathways. Besides unwinding RNA substrates for exosome-mediated degradation, MTR4 associates with RNA-binding proteins that function as adaptors in different RNA processing and decay pathways. Here, we identify and characterize the interactions of human MTR4 with a ribosome processing adaptor, NVL, and with ZCCHC8, an adaptor involved in the decay of small nuclear RNAs. We show that the unstructured regions of NVL and ZCCHC8 contain short linear motifs that bind the MTR4 arch domain in a mutually exclusive manner. These short sequences diverged from the arch-interacting motif (AIM) of yeast rRNA processing factors. Our results suggest that nuclear exosome adaptors have evolved canonical and non-canonical AIM sequences to target human MTR4 and demonstrate the versatility and specificity with which the MTR4 arch domain can recruit a repertoire of different RNA-binding proteins.
Metadaten
Author:Mahesh Lingaraju, Dennis Johnsen, Andreas SchlundtORCiDGND, Lukas M. Langer, Jérôme Basquin, Michael SattlerORCiD, Torben Heick Jensen, Sebastian FalkORCiDGND, Elena Conti
URN:urn:nbn:de:hebis:30:3-508706
DOI:https://doi.org/10.1038/s41467-019-11339-x
ISSN:2041-1723
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/31358741
Parent Title (English):Nature Communications
Publisher:Nature Publishing Group UK
Place of publication:[London]
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/07/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/09/05
Tag:NMR spectroscopy; RNA; RNA decay; X-ray crystallography
Volume:10
Issue:1, Art. 3393
Page Number:11
First Page:1
Last Page:11
Note:
Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
HeBIS-PPN:453739725
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0