Jan Valečka, Voahirana Camosseto, David G. McEwan, Zhuangzhuang Liu, Eva Strock, Catarina R. Almeida, Bing Su, Ivan Đikić, Yinming Liang, Evelina Gatti, Philippe Pierre
- We report here that RUFY4, a newly characterized member of the ‘RUN and FYVE domain-containing’ family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
Metadaten| Author: | Jan Valečka, Voahirana CamossetoORCiD, David G. McEwanORCiD, Zhuangzhuang Liu, Eva Strock, Catarina R. AlmeidaORCiD, Bing Su, Ivan ĐikićORCiDGND, Yinming Liang, Evelina GattiORCiD, Philippe PierreORCiD |
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| URN: | urn:nbn:de:hebis:30:3-629166 |
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| DOI: | https://doi.org/10.1098/rsos.202333 |
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| ISSN: | 2054-5703 |
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| Parent Title (English): | Royal Society Open Science |
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| Publisher: | Royal Soc. Publ. |
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| Place of publication: | London |
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| Document Type: | Article |
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| Language: | English |
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| Date of Publication (online): | 2021/07/14 |
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| Date of first Publication: | 2021/07/14 |
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| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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| Release Date: | 2023/06/14 |
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| Tag: | LPS; RUFY; Skp; alveolar macrophages; mitophagy |
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| Volume: | 8 |
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| Issue: | 7 |
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| Page Number: | 15 |
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| First Page: | 1 |
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| Last Page: | 15 |
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| Note: | The project and J.V. were supported by grants from l'Agence Nationale de la Recherche (ANR) ‘DCBIOL Labex ANR-11-LABEX-0043’, ‘INFORM Labex ANR-11-LABEX-0054’ funded by the ‘Investissements d'Avenir’ French government programme. This work was also financially supported by the project PTDC/BIA-CEL/28791/2017 and POCI-01-0145-FEDER-028791, as well as POCI-01-0145-FEDER-030882 and PTDC/BIA-MOL/30882/2017, funded by FEDER, through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES. |
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| Note: | Data accessibility
The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD026728. |
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| Note: | Electronic supplementary material is available online at https://doi.org/10.6084/m9.figshare.c.5509720. |
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| HeBIS-PPN: | 510041442 |
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| Institutes: | Medizin / Medizin |
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| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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| Sammlungen: | Universitätspublikationen |
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| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
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