Sensitizer-enhanced two-photon patterning of biomolecules in photoinstructive hydrogels
- Photoresponsive hydrogels can be employed to coordinate the organization of proteins in three dimensions (3D) and thus to spatiotemporally control their physiochemical properties by light. However, reversible and user-defined tethering of proteins and protein complexes to biomaterials pose a considerable challenge as this is a cumbersome process, which, in many cases, does not support the precise localization of biomolecules in the z direction. Here, we report on the 3D patterning of proteins with polyhistidine tags based on in-situ two-photon lithography. By exploiting a two-photon activatable multivalent chelator head, we established the protein mounting of hydrogels with micrometer precision. In the presence of photosensitizers, a substantially enhanced two-photon activation of the developed tool inside hydrogels was detected, enabling the user-defined 3D protein immobilization in hydrogels with high specificity, micrometer-scale precision, and under mild light doses. Our protein-binding strategy allows the patterning of a wide variety of proteins and offers the possibility to dynamically modify the biofunctional properties of materials at defined subvolumes in 3D.
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| Author: | Heike KrügerGND, Marvin AsidoORCiDGND, Josef WachtveitlORCiDGND, Robert TampéORCiDGND, Ralph WienekeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-697800 |
| DOI: | https://doi.org/10.1038/s43246-022-00230-w |
| ISSN: | 2662-4443 |
| Parent Title (English): | Communications materials |
| Publisher: | Springer Nature |
| Place of publication: | London |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2022/02/14 |
| Date of first Publication: | 2022/02/14 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/12/04 |
| Tag: | Biomaterials – proteins; Chemical tools; Multiphoton microscopy |
| Volume: | 3 |
| Issue: | art. 9 |
| Article Number: | 9 |
| Page Number: | 12 |
| First Page: | 1 |
| Last Page: | 12 |
| Note: | This research was supported by the German Research Foundation (GRK 1986 to R.W. and R.T.), the LOEWE program (DynaMem A03 to R.W. and R.T.), the Volkswagen Foundation (Az. 96 498 to R.W. and Az. 96 496 to R.T.) and the Reinhart Koselleck Project (TA 157/12–1 to R.T.). |
| Note: | Open Access funding enabled and organized by Projekt DEAL. |
| HeBIS-PPN: | 516359908 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
