Enzymatische Konfigurationsbestimmung von Nucleosiden
- It is possible to determine the anomeric configuration of nucleosides by a simple, spectrophotometric assay, using the nucleoside phosphorylase activity of cell-free extracts from E. coli. β-nucleo-sides are split, a-anomers remain unchanged. For a single estimation 20-40 µg of nucleoside are required. 6-Azauracil- and 8-azaguanine-β-ᴅ-riboside and some nucleoside phosphates are resistant, a fact, which is of interest in view of the specificity of nucleoside phosphorylases.
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| Author: | Edgar LodemannGND, Adolf WackerGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-748692 |
| DOI: | https://doi.org/10.1515/znb-1967-0109 |
| ISSN: | 1865-7117 |
| ISSN: | 0932-0776 |
| Parent Title (German): | Zeitschrift für Naturforschung, B |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | German |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1967 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/07/27 |
| Volume: | 22 |
| Issue: | 1 |
| Page Number: | 3 |
| First Page: | 42 |
| Last Page: | 44 |
| HeBIS-PPN: | 512590796 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
