The cytochrome c oxidase from Paracoccus denitrificans does not change the metal center ligation upon reduction
- Cytochrome c oxidase catalyzes the reduction of oxygen to water. This process is accompanied by the vectorial transport of protons across the mitochondrial or bacterial membrane (“proton pumping”). The mechanism of proton pumping is still a matter of debate. Many proposed mechanisms require structural changes during the reaction cycle of cytochrome c oxidase. Therefore, the structure of the cytochrome c oxidase was determined in the completely oxidized and in the completely reduced states at a temperature of 100 K. No ligand exchanges or other major structural changes upon reduction of the cytochrome coxidase from Paracoccus denitrificans were observed. The three histidine CuB ligands are well defined in the oxidized and in the reduced states. These results are hardly compatible with the “histidine cycle” mechanisms formulated previously.
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| Author: | Axel HarrengaGND, Hartmut MichelORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-758640 |
| DOI: | https://doi.org/10.1074/jbc.274.47.33296 |
| ISSN: | 0021-9258 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/10559205 |
| Parent Title (English): | Journal of biological chemistry |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Year of first Publication: | 1999 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/11/14 |
| Volume: | 274 |
| Issue: | 47 |
| Page Number: | 4 |
| First Page: | 33296 |
| Last Page: | 33299 |
| HeBIS-PPN: | 516504266 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
