Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium
- Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm formation and DNA uptake (natural transformation). Here, we use cryo-electron microscopy and mass spectrometry to show that the bacterium Thermus thermophilus produces two forms of type IV pilus ("wide" and "narrow"), differing in structure and protein composition. Wide pili are composed of the major pilin PilA4, while narrow pili are composed of a so-far uncharacterized pilin which we name PilA5. Functional experiments indicate that PilA4 is required for natural transformation, while PilA5 is important for twitching motility.
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| Verfasserangaben: | Alexander Neuhaus, Muniyandi Selvaraj, Ralf SalzerORCiDGND, Julian David LangerORCiDGND, Kerstin KruseGND, Lennart Kirchner, Kelly Sanders, Bertram Daum, Beate AverhoffORCiD, Vicki A. M. GoldORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-535314 |
| Titel des übergeordneten Werkes (Englisch): | Nature Communications |
| Verlag: | Nature Publishing Group UK |
| Verlagsort: | [London] |
| Dokumentart: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Fertigstellung: | 2020 |
| Datum der Erstveröffentlichung: | 06.05.2020 |
| Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Datum der Freischaltung: | 11.05.2020 |
| Freies Schlagwort / Tag: | Bacterial structural biology; Cellular microbiology; Cryoelectron microscopy |
| Jahrgang: | 11 |
| Ausgabe / Heft: | 1, Art. 2231 |
| Seitenzahl: | 13 |
| Erste Seite: | 1 |
| Letzte Seite: | 13 |
| Bemerkung: | Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| HeBIS-PPN: | 465569102 |
| Institute: | Biowissenschaften / Biowissenschaften |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Angeschlossene und kooperierende Institutionen / MPI für Hirnforschung | |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Lizenz (Deutsch): |  Creative Commons - Namensnennung 4.0 |
