Quantitative analysis of Gria1, Gria2, Dlg1 and Dlg4 expression levels in hippocampus following forced swim stress in mice
- AMPA receptors and interacting proteins are importantly involved in mediating stress-dependent plasticity. Previously we reported that GluA1-containing AMPA receptors and their interaction with PDZ-proteins are required for the experience-dependent expression of behavioral despair in the forced swim test. However, it is unclear if the expression of GluA1-containing AMPA receptors is affected by this type of behavior. Here we investigated in wild type mice, whether hippocampal gene or protein levels of GluA1 or associated PDZ proteins is altered following forced swim stress. We show that expression of Dlg4 (the gene coding for PSD-95) was strongly reduced after two days of forced swimming. In contrast, levels of Dlg1, Gria1, and Gria2 (coding for SAP97, GluA1, and GluA2 respectively) were not affected after one or two days of forced swimming. The changes in gene expression largely did not translate to the protein level. These findings indicate a limited acute effect of forced swim stress on the expression of the investigated targets and suggest that the acute involvement of GluA1-containing AMPA receptors tor forced swim behavior is a result of non-genomic mechanisms.
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| Verfasserangaben: | Florian FreudenbergORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-511171 |
| DOI: | https://doi.org/10.1038/s41598-019-50689-w |
| ISSN: | 2045-2322 |
| Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/31575955 |
| Titel des übergeordneten Werkes (Englisch): | Scientific reports |
| Verlag: | Macmillan Publishers Limited, part of Springer Nature |
| Verlagsort: | [London] |
| Dokumentart: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Fertigstellung: | 2019 |
| Datum der Erstveröffentlichung: | 01.10.2019 |
| Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Datum der Freischaltung: | 16.10.2019 |
| Freies Schlagwort / Tag: | Molecular neuroscience; Stress and resilience |
| Jahrgang: | 9 |
| Ausgabe / Heft: | 1, Art. 14060 |
| Seitenzahl: | 8 |
| Erste Seite: | 1 |
| Letzte Seite: | 8 |
| Bemerkung: | Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| HeBIS-PPN: | 455465789 |
| Institute: | Medizin / Medizin |
| DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Open-Access-Publikationsfonds: | Medizin |
| Lizenz (Deutsch): |  Creative Commons - Namensnennung 4.0 |
