TY  - JOUR
A1  - Banat, Gamal-André
A1  - Tretyn, Aleksandra
A1  - Pullamsetti, Soni Savai
A1  - Wilhelm, Jochen
A1  - Weigert, Andreas
A1  - Olesch, Catherine
A1  - Ebel, Katharina
A1  - Stiewe, Thorsten
A1  - Grimminger, Friedrich
A1  - Seeger, Werner
A1  - Fink, Ludger
A1  - Savai, Rajkumar
T1  - Immune and inflammatory cell composition of human lung cancer stroma
T2  - PLoS One
N2  - Recent studies indicate that the abnormal microenvironment of tumors may play a critical role in carcinogenesis, including lung cancer. We comprehensively assessed the number of stromal cells, especially immune/inflammatory cells, in lung cancer and evaluated their infiltration in cancers of different stages, types and metastatic characteristics potential. Immunohistochemical analysis of lung cancer tissue arrays containing normal and lung cancer sections was performed. This analysis was combined with cyto-/histomorphological assessment and quantification of cells to classify/subclassify tumors accurately and to perform a high throughput analysis of stromal cell composition in different types of lung cancer. In human lung cancer sections we observed a significant elevation/infiltration of total-T lymphocytes (CD3+), cytotoxic-T cells (CD8+), T-helper cells (CD4+), B cells (CD20+), macrophages (CD68+), mast cells (CD117+), mononuclear cells (CD11c+), plasma cells, activated-T cells (MUM1+), B cells, myeloid cells (PD1+) and neutrophilic granulocytes (myeloperoxidase+) compared with healthy donor specimens. We observed all of these immune cell markers in different types of lung cancers including squamous cell carcinoma, adenocarcinoma, adenosquamous cell carcinoma, small cell carcinoma, papillary adenocarcinoma, metastatic adenocarcinoma, and bronchioloalveolar carcinoma. The numbers of all tumor-associated immune cells (except MUM1+ cells) in stage III cancer specimens was significantly greater than those in stage I samples. We observed substantial stage-dependent immune cell infiltration in human lung tumors suggesting that the tumor microenvironment plays a critical role during lung carcinogenesis. Strategies for therapeutic interference with lung cancer microenvironment should consider the complexity of its immune cell composition.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/39041
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-390411
SN  - 1932-6203
N1  - Copyright: © 2015 Banat et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
VL  - 10
IS  - (9): e0139073
SP  - 1
EP  - 21
PB  - PLoS
CY  - Lawrence, Kan.
ER  -