TY - JOUR A1 - Müller-McNicoll, Michaela A1 - Botti, Valentina A1 - de Jesus Domingues, Antonio M. A1 - Brandl, Holger A1 - Schwich, Oliver Daniel A1 - Steiner, Christina Michaela A1 - Curk, Tomaz A1 - Poser, Ina A1 - Zarnack, Katharina A1 - Neugebauer, Karla M. T1 - SR proteins are NXF1 adaptors that link alternative RNA processing to mRNA export T2 - Genes & Development N2 - Nuclear export factor 1 (NXF1) exports mRNA to the cytoplasm after recruitment to mRNA by specific adaptor proteins. How and why cells use numerous different export adaptors is poorly understood. Here we critically evaluate members of the SR protein family (SRSF1-7) for their potential to act as NXF1 adaptors that couple pre-mRNA processing to mRNA export. Consistent with this proposal, >1000 endogenous mRNAs required individual SR proteins for nuclear export in vivo. To address the mechanism, transcriptome-wide RNA-binding profiles of NXF1 and SRSF1-7 were determined in parallel by individual-nucleotide-resolution UV cross-linking and immunoprecipitation (iCLIP). Quantitative comparisons of RNA-binding sites showed that NXF1 and SR proteins bind mRNA targets at adjacent sites, indicative of cobinding. SRSF3 emerged as the most potent NXF1 adaptor, conferring sequence specificity to RNA binding by NXF1 in last exons. Interestingly, SRSF3 and SRSF7 were shown to bind different sites in last exons and regulate 3' untranslated region length in an opposing manner. Both SRSF3 and SRSF7 promoted NXF1 recruitment to mRNA. Thus, SRSF3 and SRSF7 couple alternative splicing and polyadenylation to NXF1-mediated mRNA export, thereby controlling the cytoplasmic abundance of transcripts with alternative 3' ends. KW - NXF1 KW - SR protein KW - SRSF3 KW - SRSF7 KW - alternative 3′ end processing KW - iCLIP KW - mRNA export Y1 - 2016 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/41289 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-412893 SN - 0890-9369 N1 - © 2016 Müller-McNicoll et al. This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. VL - 30 IS - 5 SP - 553 EP - 556 PB - Cold Spring Harbor Laboratory Press ER -