TY  - JOUR
A1  - Beyer, Kim
A1  - Nikfarjam, Frangis
A1  - Butting, Manuel
A1  - Meissner, Markus
A1  - König, Anke
A1  - Bosca, Ana Ramirez
A1  - Kaufmann, Roland
A1  - Heidemann, Detlef
A1  - Bernd, August
A1  - Kippenberger, Stefan
A1  - Zöller, Nadja Nicole
T1  - Photodynamic treatment of oral squamous cell carcinoma cells with low curcumin concentrations
T2  - Journal of cancer
N2  - Objective: Curcumin is known for its anti-oxidative, anti-inflammatory and anti-tumorigenic qualities at concentrations ranging from 3.7µg/ml to 55µg/ml. Therefore it is pre-destined for tumour therapy. Due to high oral doses that have to be administered and the low bioavailability of curcumin new therapy concepts have to be developed. One of these therapy concepts is the combination of low curcumin concentrations and UVA or visible light. Aim of our study was to investigate the influence of this treatment regime on oral squamous cell carcinoma cells.
Materials and Methods: A human oral squamous cell carcinoma cell line (HN) was pre-incubated with low curcumin concentrations (0.01µg/ml to 1µg/ml). Thereafter cell cultures were either left un-irradiated or were irradiated either with 1J/cm2 UVA or for 5min with visible light. Quantitative analysis of proliferation, membrane integrity, oxidative potential and DNA fragmentation were done.
Results: It could be shown that low curcumin concentrations neither influenced proliferation, nor cell morphology, nor cell integrity nor apoptosis. When combining these curcumin concentrations with UVA or visible light irradiation cell proliferation as well as development of reactive oxygen species was reduced whereas DNA fragmentation was increased. Concentration as well as light entity specific effects could be observed.
Conclusions: The present findings substantiate the potential of the combination of low curcumin concentrations and light as a new therapeutic concept to increase the efficacy of curcumin in the treatment of cancer of the oral mucosa.
KW  - head and neck cancer
KW  - curcumin
KW  - apoptosis
KW  - proliferation
KW  - UVA
KW  - VIS
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44251
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-442514
SN  - 1837-9664
N1  - © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
VL  - 8
IS  - 7
SP  - 1271
EP  - 1283
PB  - Ivyspring Internat. Publ.
CY  - Wyoming, NSW
ER  -