Direct estimation of the spontaneous mutation rate by short-term mutation accumulation lines in Chironomus riparius
- Mutations are the ultimate basis of evolution, yet their occurrence rate is known only for few species. We directly estimated the spontaneous mutation rate and the mutational spectrum in the nonbiting midge C. riparius with a new approach. Individuals from ten mutation accumulation lines over five generations were deep genome sequenced to count de novo mutations that were not present in a pool of F1 individuals, representing parental genotypes. We identified 51 new single site mutations of which 25 were insertions or deletions and 26 single nucleotide mutations. This shift in the mutational spectrum compared to other organisms was explained by the high A/T content of the species. We estimated a haploid mutation rate of 2.1 × 10−9 (95% confidence interval: 1.4 × 10−9 – 3.1 × 10-9) that is in the range of recent estimates for other insects and supports the drift barrier hypothesis. We show that accurate mutation rate estimation from a high number of observed mutations is feasible with moderate effort even for nonmodel species.
Author: | Ann-Marie OppoldORCiDGND, Markus PfenningerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-451761 |
DOI: | https://doi.org/10.1002/evl3.8 |
ISSN: | 2056-3744 |
Parent Title (English): | Evolution letters |
Publisher: | Wiley |
Place of publication: | Chichester |
Document Type: | Article |
Language: | English |
Year of Completion: | 2017 |
Date of first Publication: | 2017/05/11 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/12/05 |
Tag: | Diptera; genomic base composition; insect; mutational spectrum bias |
Volume: | 1 |
Issue: | 2 |
Page Number: | 7 |
First Page: | 86 |
Last Page: | 92 |
Note: | © 2017 The Author(s). Evolution Letters published by Wiley Periodicals, Inc. on behalf of Society for the Study of Evolution (SSE) and European Society for Evolutionary Biology (ESEB). This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
HeBIS-PPN: | 427941261 |
Institutes: | Biowissenschaften / Institut für Ökologie, Evolution und Diversität |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie) |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Biowissenschaften |
Licence (German): | Creative Commons - Namensnennung 4.0 |