Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors

Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human
Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.
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Metadaten
Author:Sandra Siegert, Sonja Thaler, Ralf Wagner, Barbara S. Schnierle
URN:urn:nbn:de:hebis:30-26194
URL:http://www.aidsrestherapy.com/content/2/1/7
Parent Title (German):AIDS Research and Therapy
Document Type:Article
Language:English
Year of Completion:2005
Date of first Publication:2005/09/12
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2006/05/08
Tag:HIV-1 ; MLV/HIV-1 ; pseudotyped vectors
Volume:2
Issue:7
Pagenumber:6
Note:
© 2005 Siegert et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:AIDS Res Ther. 2005; 2: 7. http://www.aidsrestherapy.com/content/2/1/7
HeBIS PPN:189203684
Institutes:Georg-Speyer-Haus
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 2.0

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