Chemically induced photoswitching of fluorescent probes : a general concept for super-resolution microscopy
- We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.
| Author: | Ulrike EndesfelderORCiDGND, Sebastian MalkuschORCiDGND, Benjamin FlottmannGND, Justine Mondry, Piotr Liguzinski, Peter J. Verveer, Mike HeilemannORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-348104 |
| DOI: | https://doi.org/10.3390/molecules16043106 |
| ISSN: | 1420-3049 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/21490558 |
| Parent Title (English): | Molecules |
| Publisher: | Molecular Diversity Preservation International |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2011/04/13 |
| Date of first Publication: | 2011/04/13 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2014/10/27 |
| Tag: | PALM; dSTORM; fluorescent proteins; photoswitchable organic fluorophores; super-resolution |
| Volume: | 16 |
| Issue: | 4 |
| Page Number: | 13 |
| First Page: | 3106 |
| Last Page: | 3118 |
| Note: | © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| HeBIS-PPN: | 366619772 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 3.0 |
