Nueva técnica de microscopía electrónica de barrido para el estudio de Coccoidea (Insecta: Hemiptera)
- A new protocol to prepare material of Coccoidea (Insecta: Hemiptera) for observation with scanning electron microscopy is presented. This technique consists of cleaning samples (stored in 96% alcohol) with acetone and xylene, taking them directly to the hydration step, and subsequently dehydrating them in alcohol. Fixation in glutaraldehyde and post-fi xation in osmium tetroxide are omitted. The cleaning of the samples and the correct critical point dryer are very important.
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| Author: | Andrea Saracho Bottero, Lucía E. Claps |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-396957 |
| ISSN: | 1942-1354 |
| Parent Title (English): | Insecta mundi : a journal of world insect systematics |
| Series (Serial Number): | Insecta Mundi (0446) |
| Publisher: | Center for Systematic Entomology |
| Place of publication: | Gainesville, Fla. |
| Document Type: | Part of Periodical |
| Language: | English |
| Date of Publication (online): | 2016/05/19 |
| Year of first Publication: | 2015 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2016/05/19 |
| Tag: | Dactylopiidae; Pseudococcidae; SEM |
| Page Number: | 8 |
| First Page: | 1 |
| Last Page: | 5 |
| HeBIS-PPN: | 380636956 |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie) |
| Sammlungen: | Sammlung Biologie / Sondersammelgebiets-Volltexte |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell 3.0 |
