Alexander Strassman, Frank Schnütgen, Qi Dai, Jennifer C. Jones, Angela C. Gomez, Lenore Pitstick, Nathan E. Holton, Russell Moskal, Erin R. Leslie, Harald von Melchner, David R. Beier, Bryan C. Bjork
- Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1IREScre deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.
MetadatenAuthor: | Alexander Strassman, Frank SchnütgenORCiDGND, Qi Dai, Jennifer C. Jones, Angela C. Gomez, Lenore Pitstick, Nathan E. Holton, Russell Moskal, Erin R. Leslie, Harald von Melchner, David R. Beier, Bryan C. Bjork |
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URN: | urn:nbn:de:hebis:30:3-439128 |
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DOI: | https://doi.org/10.1242/dmm.029561 |
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ISSN: | 1754-8411 |
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ISSN: | 1754-8403 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/28424158 |
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Parent Title (English): | Disease models & mechanisms |
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Publisher: | Company of Biologists Limited |
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Place of publication: | Cambridge |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2017/10/26 |
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Date of first Publication: | 2017/07/05 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2017/10/26 |
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Tag: | Cleft palate; Conditional gene trap; Mandible; Micrognathia; Pierre Robin sequence |
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Volume: | 10 |
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Issue: | 7 |
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Page Number: | 14 |
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First Page: | 909 |
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Last Page: | 922 |
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Note: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
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HeBIS-PPN: | 453772013 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 3.0 |
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