A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1
- The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. Here, we report the crystal structure of the UBZ domain of Y-family DNA polymerase (pol) η and the crystal structure of the complex between the UBZ domain of Werner helicase-interacting protein 1 (WRNIP1) and ubiquitin, crystallized using the GFP fusion technique. In contrast to the pol η UBZ, which has been proposed to bind ubiquitin via its C-terminal α-helix, ubiquitin binds to a novel surface of WRNIP1 UBZ composed of the first β-strand and the C-terminal α-helix. In addition, we report the structure of the tandem UBZ domains of Tax1-binding protein 1 (TAX1BP1) and show that the second UBZ of TAX1BP1 binds ubiquitin, presumably in a manner similar to that of WRNIP1 UBZ. We propose that UBZ domains can be divided into at least two different types in terms of the ubiquitin-binding surfaces: the pol η type and the WRNIP1 type.
Author: | Nobuhiro Suzuki, Ahmed Rohaim, Ryuichi Kato, Ivan ĐikićORCiDGND, Soichi Wakatsuki, Masato Kawasaki |
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URN: | urn:nbn:de:hebis:30:3-443039 |
DOI: | https://doi.org/10.1111/febs.13734 |
ISSN: | 1742-4658 |
ISSN: | 1742-464X |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27062441 |
Parent Title (English): | The FEBS journal |
Publisher: | Wiley-Blackwell |
Place of publication: | Oxford [u. a.] |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2017/06/26 |
Year of first Publication: | 2016 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/06/26 |
Tag: | TAX1BP1; WRNIP1; green fluorescent protein; pol g; ubiquitin-binding zinc finger |
Volume: | 283 |
Issue: | 11 |
Page Number: | 14 |
First Page: | 2004 |
Last Page: | 2017 |
Note: | © 2016 Federation of European Biochemical Societies |
HeBIS-PPN: | 423894889 |
Institutes: | Medizin / Medizin |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Archivex. zur Lesesaalplatznutzung § 52b UrhG |