Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins

  • We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.

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Author:Anika Raulf, Christoph Klaus SpahnORCiDGND, Patrick Zessin, Kieran Finan, Stefan Bernhardt, Alexander HeckelORCiDGND, Mike HeilemannORCiDGND
URN:urn:nbn:de:hebis:30:3-450166
DOI:https://doi.org/10.1039/C4RA01027B
ISSN:2046-2069
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/25580242
Parent Title (English):RSC Advances
Publisher:RSC Publishing
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2014
Date of first Publication:2014/06/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2017/12/14
Volume:4
Issue:57
Page Number:5
First Page:30462
Last Page:30466
Note:
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
HeBIS-PPN:430463235
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0