PhostagTM-gel retardation and in situ thylakoid kinase assay for determination of chloroplast protein phosphorylation targets
- The chloroplast phosphorylation network is important for posttranslational regulation of photosynthetic complexes, gene expression and metabolic pathways. In mass-spectrometric analyses a lot of putative phosphorylation targets have been found but these data need to be confirmed and brought into a physiological context. Here, we present a current protocol to quantify the phosphorylation state of thylakoid proteins and an in situ method to verify putative substrates for thylakoid associated kinases.
Author: | Yuliya Dytyuk, Falko Flügge, Olaf Czarnecki, Bernhard Grimm, Lars Dietzel |
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URN: | urn:nbn:de:hebis:30:3-451113 |
URL: | http://zs.thulb.uni-jena.de/receive/jportal_jparticle_00433957 |
Parent Title (English): | Endocytobiosis and cell research |
Publisher: | Thüringer Univ.- und Landesbibliothek |
Place of publication: | Jena |
Document Type: | Article |
Language: | English |
Year of Completion: | 2016 |
Date of first Publication: | 2016/04/21 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/11/30 |
Tag: | chloroplast phosphorylation network; gel retardation; kinase protein interaction; posttranslational regulation of photosynthesis; thylakoid kinases |
Volume: | 27 |
Issue: | 2 |
Page Number: | 9 |
First Page: | 62 |
Last Page: | 70 |
HeBIS-PPN: | 425147231 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Biowissenschaften |
Licence (German): | Deutsches Urheberrecht |