PTCH-1 and MDM2 expression in ameloblastoma from a West African sub-population: implication for chemotherapeutics
- Introduction: ameloblastoma is a slow growing, painless odontogenic swelling which can attain sizes that result in severe deformities of the craniofacial complex. It is the most commonly encountered odontogenic tumor in Nigeria. Surgical intervention is currently the method of treatment; however identification of altered molecular pathways may inform chemotherapeutic potential. The Protein Patched homolog 1 (PTCH-1) is overexpressed in ameloblastoma. Also, mutation in the MDM2 gene can reduce the tumor suppressor function of p53 and promote ameloblastoma growth. No study however has characterized the molecular profile of African cases of ameloblastoma with a view to developing chemotherapeutic alternatives. The objective was to characterize the PTCH-1 genetic profile of Ameloblastoma in Nigerian patients as a first step in investigating its potential for chemotherapeutic intervention. Methods: twenty-eight FFPE blocks of ameloblastoma cases from Nigerian patients were prepared for antibody processing to PTCH-1 (Polyclonal Anti-PTCH antibody ab39266) and MDM2 (Monoclonal Anti-MDM2 antibody (2A10) ab16895). Cytoplasmic brown staining was considered as positive for PTCH while nuclear staining was positive for MDM2. Results: moderate and strong expressions for PTCH in ameloblast and stellate reticulum were 78.6% and 60.7% respectively. Only 3 (10.7%) cases expressed MDM2. Conclusion: the importance of our study is that it supports, in theory, anti-PTCH/SHH chemotherapeutics for Nigerian ameloblastoma cases and also infers the possible additional use of anti-p53 agents.
| Author: | Samuel Ebele UdeaborORCiDGND, Akinyele Olumuyiwa AdisaORCiD, Ahmed Oluwatoyin Lawal, Mike Barbeck, Patrick Booms, Robert Alexander SaderORCiDGND, Shahram Michael GhanaatiORCiDGND |
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| URN: | urn:nbn:de:hebis:30:3-403373 |
| URL: | http://www.panafrican-med-journal.com/content/article/20/140/full/ |
| DOI: | https://doi.org/10.11604/pamj.2015.20.140.5869 |
| ISSN: | 1937-8688 |
| Parent Title (English): | Pan African Medical Journal |
| Publisher: | African Field Epidemiology Network (AFENET) |
| Place of publication: | Kampala, Uganda |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2015/02/17 |
| Date of first Publication: | 2015/02/17 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2016/06/02 |
| Tag: | MdM2; PTCH-1; ameloblastoma; chemotherapeutics |
| Volume: | 20 |
| Issue: | 140 |
| Page Number: | 8 |
| First Page: | 1 |
| Last Page: | 8 |
| Note: | © Shahram Ghanaati et al. The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| HeBIS-PPN: | 428737161 |
| Institutes: | Medizin / Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 2.0 |
