Latest documents http://publikationen.ub.uni-frankfurt.de/index/index/ Thu, 14 Feb 2013 15:26:07 +0100 Thu, 14 Feb 2013 15:26:07 +0100 http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/28879 Two new and five known oxazoles were identified from two different Pseudomonas strains in addition to the known pyrones pseudopyronine A and B. Labeling experiments confirmed their structures and gave initial evidence for a novel biosynthesis pathway of these natural oxazoles. In order to confirm their structure, they were synthesized, which also allowed tests of their bioactivity. Additionally, the bioactivities of the synthesis intermediates were also investigated revealing interesting biological activities for several compounds despite their overall simple structures. Florian Grundmann; Veronika Dill; Andrea Dowling; Aunchalee Thanwisai; Edna Bode; Narisara Chantratita; Richard ffrench-Constant; Helge Björn Bode article http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/28879 Thu, 14 Feb 2013 15:26:07 +0100 http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/28888 Janthinobacteria commonly form biofilms on eukaryotic hosts and are known to synthesize antibacterial and antifungal compounds. Janthinobacterium sp. HH01 was recently isolated from an aquatic environment and its genome sequence was established. The genome consists of a single chromosome and reveals a size of 7.10 Mb, being the largest janthinobacterial genome so far known. Approximately 80% of the 5,980 coding sequences (CDSs) present in the HH01 genome could be assigned putative functions. The genome encodes a wealth of secretory functions and several large clusters for polyketide biosynthesis. HH01 also encodes a remarkable number of proteins involved in resistance to drugs or heavy metals. Interestingly, the genome of HH01 apparently lacks the N-acylhomoserine lactone (AHL)-dependent signaling system and the AI-2-dependent quorum sensing regulatory circuit. Instead it encodes a homologue of the Legionella- and Vibrio-like autoinducer (lqsA/cqsA) synthase gene which we designated jqsA. The jqsA gene is linked to a cognate sensor kinase (jqsS) which is flanked by the response regulator jqsR. Here we show that a jqsA deletion has strong impact on the violacein biosynthesis in Janthinobacterium sp. HH01 and that a jqsA deletion mutant can be functionally complemented with the V. cholerae cqsA and the L. pneumophila lqsA genes. Claudia Hornung; Anja Poehlein; Frederike S. Haack; Martina Schmidt; Katja Dierking; Andrea Pohlen; Hinrich Schulenburg; Melanie Blokesch; Laure Plener; Kirsten Jung; Andreas Bonge; Ines Krohn-Molt; Christian Utpatel; Gabriele Timmermann; Eva Spieck; Andreas Pommerening-Röser; Edna Bode; Helge Björn Bode; Rolf Daniel; Christel Schmeisser; Wolfgang R. Streit article http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/28888 Wed, 13 Feb 2013 10:43:42 +0100 http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/23734 Members of the genus Xenorhabdus are entomopathogenic bacteria that associate with nematodes. The nematode-bacteria pair infects and kills insects, with both partners contributing to insect pathogenesis and the bacteria providing nutrition to the nematode from available insect-derived nutrients. The nematode provides the bacteria with protection from predators, access to nutrients, and a mechanism of dispersal. Members of the bacterial genus Photorhabdus also associate with nematodes to kill insects, and both genera of bacteria provide similar services to their different nematode hosts through unique physiological and metabolic mechanisms. We posited that these differences would be reflected in their respective genomes. To test this, we sequenced to completion the genomes of Xenorhabdus nematophila ATCC 19061 and Xenorhabdus bovienii SS-2004. As expected, both Xenorhabdus genomes encode many anti-insecticidal compounds, commensurate with their entomopathogenic lifestyle. Despite the similarities in lifestyle between Xenorhabdus and Photorhabdus bacteria, a comparative analysis of the Xenorhabdus, Photorhabdus luminescens, and P. asymbiotica genomes suggests genomic divergence. These findings indicate that evolutionary changes shaped by symbiotic interactions can follow different routes to achieve similar end points. John M. Chaston; Garret Suen; Sarah L. Tucker; Aaron W. Andersen; Archna Bhasin; Edna Bode; Helge Björn Bode; Alexander Oliver Brachmann; Charles E. Cowles; Kimberly N. Cowles; Creg Darby; Limaris de Léon; Kevin Drace; Zijin Du; Alain Givaudan; Erin E. Herbert Tran; Kelsea A. Jewell; Jennifer J. Knack; Karina C. Krasomil-Osterfeld; Ryan Kukor; Anne Lanois; Phil Latreille; Nancy K. Leimgruber; Carolyn M. Lipke; Renyi Liu; Xiaojun Lu; Eric C. Martens; Pradeep R. Marri; Claudine Médigue; Megan L. Menard; Nancy M. Miller; Nydia Morales-Soto; Stacie Norton; Jean-Claude Ogier; Samantha S. Orchard; Dongjin Park; Youngjin Park; Barbara A. Qurollo; Darby Renneckar Sugar; Gregory R. Richards; Zoé Rouy; Brad Slominski; Kathryn Slominski; Holly Snyder; Brian C. Tjaden; Ransome van der Hoeven; Roy D. Welch; Cathy Wheeler; Bosong Xiang; Brad Barbazuk; Sophie Gaudriault; Brad Goodner; Steven C. Slater; Steven Forst; Barry S. Goldman; Heidi Goodrich-Blair article http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/23734 Wed, 28 Dec 2011 11:32:45 +0000