Latest documents http://publikationen.ub.uni-frankfurt.de/index/index/ Sat, 11 Dec 2010 11:14:05 +0100 Sat, 11 Dec 2010 11:14:05 +0100 http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/20243 We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose 13C-spins, (2) the (H)CPC-experiment that correlates each phosphorus with the C40 nuclei of adjacent nucleotides via J(C,P) couplings and (3) the (H)CPC-CCH-TOCSY-experiment that correlates the phosphorus nuclei with the respective C10,H10 ribose signals. The experiments were applied to two RNA hairpin structures. The current set of 13C-direct detected experiments allows direct and unambiguous assignment of the majority of the hetero nuclei and the identification of the individual ribose moieties following their sequential assignment. Thus, 13C-direct detected NMR methods constitute useful complements to the conventional 1H-detected approach for the resonance assignment of oligonucleotides that is often hindered by the limited chemical shift dispersion. The developed methods can also be applied to large deuterated RNAs. Keywords: NMR spectroscopy , Direct carbon , detection , RNA Christian Richter; Helena Kovacs; Janina Buck; Anna Wacker; Boris Fürtig; Wolfgang Bermel; Harald Schwalbe article http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/20243 Fri, 12 Nov 2010 11:14:05 +0100