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- Optimal destabilization of DNA double strands by single-nucleobase caging (2018)
- Photolabile protecting groups are widely used to trigger oligonucleotide activity. The ON/OFF‐amplitude is a critical parameter. An experimental setup has been developed to identify protecting group derivatives with superior caging properties. Bulky rests are attached to the cage moiety via Cu‐catalyzed azide–alkyne cycloaddition post‐synthetically on DNA. Interestingly, the decrease in melting temperature upon introducing o‐nitrobenzyl‐caged (NPBY‐) and diethylaminocoumarin‐cages (DEACM‐) in DNA duplexes reaches a limiting value. NMR spectroscopy was used to characterize individual base‐pair stabilities and determine experimental structures of a selected number of photocaged DNA molecules. The experimental structures agree well with structures predicted by MD simulations. Combined, the structural data indicate that once a sterically demanding group is added to generate a tri‐substituted carbon, the sterically less demanding cage moiety points towards the neighboring nucleoside and the bulkier substituents remain in the major groove.
- Beispiellose Verstärkung von Kohlenstoffsignalen in der Flüssigphasen‐NMR‐Spektroskopie (2017)
- Eine 1000‐fache Verstärkung von 13C‐Signalen in der Flüssigphasen‐NMR‐Spektroskopie wurde durch dynamische Kernpolarisation (DNP) bei hohem Magnetfeld und bei Raumtemperatur erzielt. Die früher vorhergesagte Obergrenze für Hochfeld‐DNP in flüssiger Phase wurde damit deutlich übertroffen.
