TY  - JOUR
A1  - Vandooren, Jennifer
A1  - Berghmans, Nele
A1  - Dillen, Chris
A1  - Aelst, Ilse Van
A1  - Ronsse, Isabelle
A1  - Israel, Liron Limor
A1  - Rosenberger, Ina
A1  - Kreuter, Jörg
A1  - Lellouche, Jean-Paul
A1  - Michaeli, Shulamit
A1  - Locatelli, Erica
A1  - Franchini, Mauro Comes
A1  - Aiertza, Miren K.
A1  - Sánchez-Abella, Laura
A1  - Loinaz, Iraida
A1  - Edwards, Dylan R.
A1  - Shenkman, Louis
A1  - Opdenakker, Ghislain
T1  - Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
T2  - International journal of nanomedicine
N2  - The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersensitivity reactions, and fibrosis. Here we show that artificial particles of specific sizes affect immune cell recruitment as tested in a dermal air pouch model in mice. In addition, we demonstrate that the composition of nanoparticles may influence immune cell recruitment in vivo. Aside from biophysical characterizations in terms of hydrodynamic diameter, zeta potential, concentration, and atomic concentration of metals, we show that - after first-line in vitro assays - characterization of cellular and molecular effects by dermal air pouch analysis is straightforward and should be included in the quality control of nanoparticles. We demonstrate this for innate immunological effects such as neutrophil recruitment and the production of immune-modulating matrix metalloproteases such as MMP-9; we propose the use of air pouch leukocytosis analysis as a future standard assay.
KW  - nanoparticles
KW  - biocompatibility
KW  - toxicity
KW  - air pouch
KW  - immunology
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/34194
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-341940
SN  - 1178-2013
N1  - © 2013 Vandooren et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions
VL  - 8
SP  - 4745
EP  - 4756
PB  - DOVE Medical Press
CY  - Albany, Auckland [u.a.]
ER  -