TY  - JOUR
A1  - Feldbauer, Katrin
A1  - Schlegel, Jan
A1  - Weissbecker, Juliane
A1  - Sauer, Frank
A1  - Wood, Phillip G.
A1  - Bamberg, Ernst
A1  - Terpitz, Ulrich
T1  - Optochemokine tandem for light-control of intracellular Ca2+
T2  - PLoS one
N2  - An optochemokine tandem was developed to control the release of calcium from endosomes into the cytosol by light and to analyze the internalization kinetics of G-protein coupled receptors (GPCRs) by electrophysiology. A previously constructed rhodopsin tandem was re-engineered to combine the light-gated Ca2+-permeable cation channel Channelrhodopsin-2(L132C), CatCh, with the chemokine receptor CXCR4 in a functional tandem protein tCXCR4/CatCh. The GPCR was used as a shuttle protein to displace CatCh from the plasma membrane into intracellular areas. As shown by patch-clamp measurements and confocal laser scanning microscopy, heterologously expressed tCXCR4/CatCh was internalized via the endocytic SDF1/CXCR4 signaling pathway. The kinetics of internalization could be followed electrophysiologically via the amplitude of the CatCh signal. The light-induced release of Ca2+ by tandem endosomes into the cytosol via CatCh was visualized using the Ca2+-sensitive dyes rhod2 and rhod2-AM showing an increase of intracellular Ca2+ in response to light.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/41992
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-419927
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074463
SN  - 1932-6203
N1  - Copyright: © 2016 Feldbauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 11
IS  - (10): e0165344
SP  - 1
EP  - 20
PB  - PLoS
CY  - Lawrence, Kan.
ER  -