TY  - JOUR
A1  - Herrmann, Christine
A1  - Golkaramnay, Elaheh
A1  - Inman, Elisabeth
A1  - Rome, Leonard
A1  - Volknandt, Walter
T1  - Recombinant major vault protein is targeted to neuritic tips of PC12 cells
T2  - The journal of cell biology
N2  - The major vault protein (MVP) is the predominant constituent of ubiquitous, evolutionarily conserved large cytoplasmic ribonucleoprotein particles of unknown function. Vaults are multimeric protein complexes with several copies of an untranslated RNA. Double labeling employing laser-assisted confocal microscopy and indirect immunofluorescence demonstrates partial colocalization of vaults with cytoskeletal elements in Chinese hamster ovary (CHO) and nerve growth factor (NGF)-treated neuronlike PC12 cells. Transfection of CHO and PC12 cells with a cDNA encoding the rat major vault protein containing a vesicular stomatitis virus glycoprotein epitope tag demonstrates that the recombinant protein is sorted into vault particles and targeted like endogenous MVPs. In neuritic extensions of differentiated PC12 cells, there is an almost complete overlap of the distribution of microtubules and vaults. A pronounced colocalization of vaults with filamentous actin can be seen in the tips of neurites. Moreover, in NGF-treated PC12 cells the location of vaults partially coincides with vesicular markers. Within the terminal tips of neurites vaults are located near secretory organelles. Our observations suggest that the vault particles are transported along cytoskeletal-based cellular tracks.
KW  - vaults
KW  - protein targeting
KW  - major vault protein
KW  - transport
KW  - PC12
Y1  - 1999
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45827
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-458272
SN  - 1540-8140
SN  - 0021-9525
N1  - This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
VL  - 144
IS  - 6
SP  - 1163
EP  - 1172
PB  - Rockefeller Univ. Press
CY  - New York, NY
ER  -