TY  - JOUR
A1  - Ellenrieder, Lars
A1  - Opaliński, Łukasz
A1  - Becker, Lars
A1  - Krüger, Vivien
A1  - Mirus, Oliver
A1  - Straub, Sebastian Philipp
A1  - Ebell, Katharina
A1  - Flinner, Nadine
A1  - Stiller, Sebastian
A1  - Guiard, Bernard
A1  - Meisinger, Chris
A1  - Wiedemann, Nils
A1  - Schleiff, Enrico
A1  - Wagner, Richard
A1  - Pfanner, Nikolaus
A1  - Becker, Thomas
T1  - Separating mitochondrial protein assembly and endoplasmic reticulum tethering by selective coupling of Mdm10
T2  - Nature Communications
N2  - The endoplasmic reticulum–mitochondria encounter structure (ERMES) connects the mitochondrial outer membrane with the ER. Multiple functions have been linked to ERMES, including maintenance of mitochondrial morphology, protein assembly and phospholipid homeostasis. Since the mitochondrial distribution and morphology protein Mdm10 is present in both ERMES and the mitochondrial sorting and assembly machinery (SAM), it is unknown how the ERMES functions are connected on a molecular level. Here we report that conserved surface areas on opposite sides of the Mdm10 β-barrel interact with SAM and ERMES, respectively. We generated point mutants to separate protein assembly (SAM) from morphology and phospholipid homeostasis (ERMES). Our study reveals that the β-barrel channel of Mdm10 serves different functions. Mdm10 promotes the biogenesis of α-helical and β-barrel proteins at SAM and functions as integral membrane anchor of ERMES, demonstrating that SAM-mediated protein assembly is distinct from ER-mitochondria contact sites.
KW  - Mitochondria
KW  - Proteins
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47858
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-478581
SN  - 2041-1723
N1  - Rights and permissions: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
VL  - 7
IS  - Art. 13021
SP  - 1
EP  - 14
PB  - Nature Publishing Group UK
CY  - [London]
ER  -