TY  - JOUR
A1  - Steiner, Marlene
A1  - Schneider, Lucas
A1  - Yillah, Jasmin
A1  - Gerlach, Katharina
A1  - Lausen, Olga Nikolaevna
A1  - Meyer, Annekarin
A1  - Maring, Alisa
A1  - Bönig, Halvard-Björn
A1  - Seifried, Erhard
A1  - Zörnig, Martin
A1  - Lausen, Jörn
T1  - FUSE binding protein 1 (FUBP1) expression is upregulated by T-cell acute lymphocytic leukemia protein 1 (TAL1) and required for efficient erythroid differentiation
T2  - PLoS one
N2  - During erythropoiesis, haematopoietic stem cells (HSCs) differentiate in successive steps of commitment and specification to mature erythrocytes. This differentiation process is controlled by transcription factors that establish stage- and cell type-specific gene expression. In this study, we demonstrate that FUSE binding protein 1 (FUBP1), a transcriptional regulator important for HSC self-renewal and survival, is regulated by T-cell acute lymphocytic leukaemia 1 (TAL1) in erythroid progenitor cells. TAL1 directly activates the FUBP1 promoter, leading to increased FUBP1 expression during erythroid differentiation. The binding of TAL1 to the FUBP1 promoter is highly dependent on an intact GATA sequence in a combined E-box/GATA motif. We found that FUBP1 expression is required for efficient erythropoiesis, as FUBP1-deficient progenitor cells were limited in their potential of erythroid differentiation. Thus, the finding of an interconnection between GATA1/TAL1 and FUBP1 reveals a molecular mechanism that is part of the switch from progenitor- to erythrocyte-specific gene expression. In summary, we identified a TAL1/FUBP1 transcriptional relationship, whose physiological function in haematopoiesis is connected to proper erythropoiesis.
KW  - Cell differentiation
KW  - Gene expression
KW  - Transcription factors
KW  - Erythropoiesis
KW  - Luciferase
KW  - Transcriptional control
KW  - Sequence motif analysis
KW  - Cell binding
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48687
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-486870
SN  - 1932-6203
N1  - Copyright: © 2019 Steiner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 14
IS  - (1): e0210515
SP  - 1
EP  - 22
PB  - PLoS
CY  - Lawrence, Kan.
ER  -