TY  - JOUR
A1  - Stangl, Stefan
A1  - Tontcheva, Nikoletta
A1  - Sievert, Wolfgang
A1  - Shevtsov, Maxim
A1  - Niu, Minli
A1  - Schmid, Thomas E.
A1  - Pigorsch, Steffi
A1  - Combs, Stephanie
A1  - Haller, Bernhard
A1  - Balermpas, Panagiotis
A1  - Rödel, Franz
A1  - Rödel, Claus
A1  - Fokas, Emmanouil
A1  - Krause, Mechthild
A1  - Linge, Annett
A1  - Lohaus, Fabian
A1  - Baumann, Michael
A1  - Tinhofer, Inge
A1  - Budach, Volker
A1  - Stuschke, Martin
A1  - Grosu, Anca-Ligia
A1  - Abdollahi, Amir
A1  - Debus, Jürgen
A1  - Belka, Claus
A1  - Maihöfer, Cornelius
A1  - Mönnich, David
A1  - Zips, Daniel
A1  - Multhoff, Gabriele
T1  - Heat shock protein 70 and tumor‐infiltrating NK cells as prognostic indicators for patients with squamous cell carcinoma of the head and neck after radiochemotherapy : a multicentre retrospective study of the German Cancer Consortium Radiation Oncology Group (DKTK‐ROG)
T2  - International journal of cancer
N2  - Tumor cells frequently overexpress heat shock protein 70 (Hsp70) and present it on their cell surface, where it can be recognized by pre‐activated NK cells. In our retrospective study the expression of Hsp70 was determined in relation to tumor‐infiltrating CD56+ NK cells in formalin‐fixed paraffin embedded (FFPE) tumor specimens of patients with SCCHN (N = 145) as potential indicators for survival and disease recurrence. All patients received radical surgery and postoperative cisplatin‐based radiochemotherapy (RCT). In general, Hsp70 expression was stronger, but with variable intensities, in tumor compared to normal tissues. Patients with high Hsp70 expressing tumors (scores 3–4) showed significantly decreased overall survival (OS; p = 0.008), local progression‐free survival (LPFS; p = 0.034) and distant metastases‐free survival (DMFS; p = 0.044), compared to those with low Hsp70 expression (scores 0–2), which remained significant after adjustment for relevant prognostic variables. The adverse prognostic value of a high Hsp70 expression for OS was also observed in patient cohorts with p16‐ (p = 0.001), p53‐ (p = 0.0003) and HPV16 DNA‐negative (p = 0.001) tumors. The absence or low numbers of tumor‐infiltrating CD56+ NK cells also correlated with significantly decreased OS (p = 0.0001), LPFS (p = 0.0009) and DMFS (p = 0.0001). A high Hsp70 expression and low numbers of tumor‐infiltrating NK cells have the highest negative predictive value (p = 0.00004). In summary, a strong Hsp70 expression and low numbers of tumor‐infiltrating NK cells correlate with unfavorable outcome following surgery and RCT in patients with SCCHN, and thus serve as negative prognostic markers.
KW  - Hsp70
KW  - prognostic biomarker
KW  - SCCHN
KW  - NK cells
KW  - IHC
KW  - retrospective trial
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48694
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-486947
SN  - 1097-0215
SN  - 0020-7136
N1  - This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
VL  - 142
IS  - 9
SP  - 1911
EP  - 1925
PB  - Wiley-Liss
CY  - Bognor Regis
ER  -