TY  - JOUR
A1  - Torres Núñez, Sandra
A1  - Abdullah, Zeinab
A1  - Brol, Maximilian
A1  - Hellerbrand, Claus
A1  - Fernandez, Mercedes
A1  - Fiorotto, Romina
A1  - Klein, Sabine
A1  - Königshofer, Philipp
A1  - Liedtke, Christian
A1  - Lotersztajn, Sophie
A1  - Nevzorova, Yulia
A1  - Schierwagen, Robert
A1  - Reiberger, Thomas
A1  - Uschner, Frank Erhard
A1  - Tacke, Frank
A1  - Weiskirchen, Ralf
A1  - Trebicka, Jonel
T1  - Recent advances in practical methods for liver cell biology: a short overview
T2  - International journal of molecular sciences
N2  - Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
KW  - hepatic stellate cells
KW  - hepatocellular cancer
KW  - fibrogenesis
KW  - steatosis
KW  - in vitro models
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55295
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-552956
SN  - 1422-0067
SN  - 1661-6596
N1  - © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 21
IS  - (6), 2027
SP  - 1
EP  - 19
PB  - MDPI
CY  - Basel
ER  -