TY  - JOUR
A1  - Schindler, Cora Rebecca
A1  - Woschek, Mathias
A1  - Vollrath, Jan Tilmann
A1  - Kontradowitz, Kerstin
A1  - Lustenberger, Thomas
A1  - Störmann, Philipp
A1  - Marzi, Ingo
A1  - Henrich, Dirk
T1  - miR-142-3p expression is predictive for severe traumatic brain injury (TBI) in trauma patients
T2  - International journal of molecular sciences
N2  - Background: Predictive biomarkers in biofluids are the most commonly used diagnostic method, but established markers in trauma diagnostics lack accuracy. This study investigates promisingmicroRNAs(miRNA)releasedfromaffectedtissueafterseveretraumathathavepredictive values for the effects of the injury.
Methods: A retrospective analysis of prospectively collected data and blood samples of n = 33 trauma patients (ISS≥16) is provided. Levels of miR-9-5p, -124-3p, -142-3p, -219a-5p, -338-3pand-423-3p inseverelyinjuredpatients (PT)withouttraumatic braininjury (TBI) or with severe TBI (PT + TBI) and patients with isolated TBI (isTBI) were measured within 6 h after trauma.
Results: The highest miR-423-3p expression was detected in patients with severe isTBI, followed by patients with PT + TBI, and lowest levels were found in PT patients without TBI (2−∆∆Ct,p = 0.009). ApositivecorrelationbetweenmiR-423-3plevelandincreasingAIShead (p = 0.001) and risk of mortality (RISC II, p = 0.062) in trauma patients (n = 33) was found. ROC analysis of miR-423-3p levels revealed them as statistically significant to predict the severity of brain injury in trauma patients (p = 0.006). miR-124-3p was only found in patients with severe TBI, miR-338-3p was shown in all trauma groups. miR-9-5p, miR-142-3p and miR-219a-5p could not be detected in any of the four groups. Conclusion: miR-423-3p expression is significantly elevated after isolated traumatic braininjuryandpredictableforsevereTBIinthefirsthoursaftertrauma. miR-423-3pcouldrepresent a promising new biomarker to identify severe isolated TBI.
KW  - miR-142-3p
KW  - miRNA
KW  - serum
KW  - plasma
KW  - SNORD95
KW  - traumaticbraininjury(TBI)
KW  - polytrauma
KW  - Injury Severity Score (ISS)
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55494
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-554942
N1  - © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 21
IS  - 15, art. 5381
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  -