TY  - JOUR
A1  - Verboket, René
A1  - Irrle, Tanja
A1  - Busche, Yannic
A1  - Schaible, Alexander
A1  - Schröder, Katrin
A1  - Brune, Jan C.
A1  - Marzi, Ingo
A1  - Nau, Christoph
A1  - Henrich, Dirk
T1  - Fibrous demineralized bone matrix (DBM) improves bone marrow mononuclear cell (BMC)-supported bone healing in large femoral bone defects in rats
T2  - Cells
N2  - Regeneration of large bone defects is a major objective in trauma surgery. Bone marrow mononuclear cell (BMC)-supported bone healing was shown to be efficient after immobilization on a scaffold. We hypothesized that fibrous demineralized bone matrix (DBM) in various forms with BMCs is superior to granular DBM. A total of 65 male SD rats were assigned to five treatment groups: syngenic cancellous bone (SCB), fibrous demineralized bone matrix (f-DBM), fibrous demineralized bone matrix densely packed (f-DBM 120%), DBM granules (GDBM) and DBM granules 5% calcium phosphate (GDBM5%Ca2+). BMCs from donor rats were combined with different scaffolds and placed into 5 mm femoral bone defects. After 8 weeks, bone mineral density (BMD), biomechanical stability and histology were assessed. Similar biomechanical properties of f-DBM and SCB defects were observed. Similar bone and cartilage formation was found in all groups, but a significantly bigger residual defect size was found in GDBM. High bone healing scores were found in f-DBM (25) and SCB (25). The application of DBM in fiber form combined with the application of BMCs shows promising results comparable to the gold standard, syngenic cancellous bone. Denser packing of fibers or higher amount of calcium phosphate has no positive effect.
KW  - critical-size defect
KW  - tissue engineering
KW  - BMNC
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61333
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-613332
SN  - 2073-4409
VL  - 10
IS  - 5, art. 1249
SP  - 1
EP  - 17
PB  - MDPI
CY  - Basel
ER  -