TY  - JOUR
A1  - Glogger, Marius
A1  - Spahn, Christoph Klaus
A1  - Enderlein, Jörg
A1  - Heilemann, Mike
T1  - Multi-color, bleaching-resistant super-resolution optical fluctuation imaging with oligonucleotide-based exchangeable fluorophores
T2  - Angewandte Chemie
N2  - Super-resolution optical fluctuation imaging (SOFI) is a super-resolution microscopy technique that overcomes the diffraction limit by analyzing intensity fluctuations of statistically independent emitters in a time series of images. The final images are background-free and show confocality and enhanced spatial resolution (super-resolution). Fluorophore photobleaching, however, is a key limitation for recording long time series of images that will allow for the calculation of higher order SOFI results with correspondingly increased resolution. Here, we demonstrate that photobleaching can be circumvented by using fluorophore labels that reversibly and transiently bind to a target, and which are being replenished from a buffer which serves as a reservoir. Using fluorophore-labeled short DNA oligonucleotides, we labeled cellular structures with target-specific antibodies that contain complementary DNA sequences and record the fluctuation events caused by transient emitter binding. We show that this concept bypasses extensive photobleaching and facilitates two-color imaging of cellular structures with SOFI.
KW  - DNA-PAINT
KW  - fluorescence
KW  - photobleaching
KW  - SOFI
KW  - super-resolution microscopy
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63870
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638707
SN  - 1521-3773
N1  - M.G., C.S. and M.H. acknowledge funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, Project-ID 259130777, SFB 1177). J.E. acknowledges support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy—EXC 2067/1-390729940. Open access funding enabled and organized by Projekt DEAL.
VL  - 60
IS  - 12
SP  - 6310
EP  - 6313
PB  - Wiley-VCH
CY  - Weinheim
ER  -