TY  - JOUR
A1  - Strack, Elisabeth
A1  - Rolfe, P. Alexander
A1  - Fink, Annika
A1  - Bankov, Katrin
A1  - Schmid, Tobias
A1  - Solbach, Christine
A1  - Savai, Rajkumar
A1  - Sha, Weixiao
A1  - Pradel, Leon
A1  - Hartmann, Sylvia
A1  - Brüne, Bernhard
A1  - Weigert, Andreas
T1  - Identification of tumor-associated macrophage subsets that are associated with breast cancer prognosis
T2  - Clinical and translational medicine
N2  - Background: Breast cancer is the leading cause of cancer-related deaths in women, demanding new treatment options. With the advent of immune checkpoint blockade, immunotherapy emerged as a treatment option. In addition to lymphocytes, tumor-associated macrophages exert a significant, albeit controversial, impact on tumor development. Pro-inflammatory macrophages are thought to hinder, whereas anti-inflammatory macrophages promote tumor growth. However, molecular markers to identify prognostic macrophage populations remain elusive. Methods: We isolated two macrophage subsets, from 48 primary human breast tumors, distinguished by the expression of CD206. Their transcriptomes were analyzed via RNA-Seq, and potential prognostic macrophage markers were validated by PhenOptics in tissue microarrays of patients with invasive breast cancer. Results: Normal human breast tissue contained mainly CD206+ macrophages, while increased relative amounts of CD206− macrophages were observed in tumors. The presence of CD206+ macrophages correlated with a pronounced lymphocyte infiltrate and subsets of CD206+ macrophages, expressing SERPINH1 and collagen 1, or MORC4, were unexpectedly associated with improved survival of breast cancer patients. In contrast, MHCIIhi CD206− macrophages were linked with a poor survival prognosis. Conclusion: Our data highlight the heterogeneity of tumor-infiltrating macrophages and suggest the use of multiple phenotypic markers to predict the impact of macrophage subpopulations on cancer prognosis. We identified novel macrophage markers that correlate with the survival of patients with invasive mammary carcinoma.
KW  - breast cancer
KW  - flow cytometry
KW  - macrophage
KW  - transcriptome
KW  - tumor microenvironment
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63948
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-639488
SN  - 2001-1326
N1  - This work was supported by Deutsche Krebshilfe (70114051), Deutsche Forschungsgemeinschaft (FOR 2438, TP8; SFB 1039, TP B04 and B06, GRK 2336, TP1 and 6), Wilhelm-Sander Foundation (2019.082.01), the LOEWE Center Frankfurt Cancer Institute (FCI) funded by the Hessen State Ministry for Higher Education, Research and the Arts (III L 5 - 519/03/03.001 - [0015]), and the German Cancer Consortium (DKTK).
VL  - 10
IS  - 8, art. e239
SP  - 1
EP  - 17
PB  - Wiley
CY  - Hoboken, NJ
ER  -