TY  - INPR
A1  - Tutucci, Evelina
A1  - Maekiniemi, Anna
A1  - Snoep, Jacky L.
A1  - Seiler, Markus
A1  - Rossum, Kelly van
A1  - Niekerk, David D. van
A1  - Savakis, Philipp
A1  - Zarnack, Katharina
A1  - Singer, Robert H.
T1  - Cyclin CLB2 mRNA localization determines efficient protein synthesis to orchestrate bud growth and cell cycle progression
T2  - bioRxiv
N2  - mRNA localization to subcellular compartments has been reported across all kingdoms of life and it is generally believed to promote asymmetric protein synthesis and localization. In striking contrast to previous observations, we show that in S. cerevisiae the B-type cyclin CLB2 mRNA is localized and translated in the yeast bud, while the Clb2 protein, a key regulator of mitosis progression, is concentrated in the mother nucleus. Using single-molecule RNA imaging in fixed (smFISH) and living cells (MS2 system), we show that the CLB2 mRNA is transported to the yeast bud by the She2-She3 complex, via an mRNA ZIP-code situated in the coding sequence. In CLB2 mRNA localization mutants, Clb2 protein synthesis in the bud is decreased resulting in changes in cell cycle distribution and genetic instability. Altogether, we propose that CLB2 mRNA localization acts as a sensor for bud development to couple cell growth and cell cycle progression, revealing a novel function for mRNA localization.
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/73026
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-730268
UR  - https://www.biorxiv.org/content/10.1101/2022.03.01.481833v1
IS  - 2022.03.01.481833 Version 1
ER  -