TY  - JOUR
A1  - Namgaladze, Dmitry
A1  - Kollas, Andreas
A1  - Brüne, Bernhard
T1  - Oxidized LDL attenuates apoptosis in monocytic cells by activating ERK signaling
T2  - Journal of lipid research
N2  - Low concentrations of oxidized low density lipoprotein (OxLDL) are cytoprotective for phagocytes, although the underlying mechanisms remain unclear. We investigated signaling pathways used by OxLDL to attenuate apoptosis in monocytic cells. OxLDL at 25–50 μg/ml inhibited staurosporine-induced apoptosis in THP-1 cells and mouse peritoneal macrophages, and it was cytoprotective in human primary monocytes upon serum withdrawal. Attenuated cell demise was reversed by blocking extracellular signal-regulated kinase (ERK) signaling. Translocation of cytochrome c to the cytosol was attenuated by OxLDL, which again demanded ERK signaling. Analysis of Bcl-2 family proteins revealed phosphorylation of Bad at serine 112 as well as ERK-dependent inhibition of Mcl-1 degradation. Although the formation of reactive oxygen species (ROS) is an established signal generated by OxLDL, ROS scavengers did not interfere with cell protection by OxLDL. Thus, activation of the ERK signaling pathway by OxLDL is important to protect phagocytes from apoptosis.
KW  - atherosclerosis
KW  - oxidized low density lipoprotein
KW  - reactive oxygen species
KW  - extracellular signal-regulated kinase
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76371
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-763717
SN  - 0022-2275
VL  - 49.2008
IS  - 1
SP  - 58
EP  - 65
PB  - American Society for Biochemistry and Molecular Biology
CY  - Bethesda, Md.
ER  -